Intranasal Flu Shot Candidate Spurs Strong Immune Response

An experimental single-dose, intranasal influenza vaccine candidate, was found safe and produced a durable immune response when tested in a Phase 1 study, announced the National Institutes of Health.

This study was sponsored by the National Institute of Allergy and Infectious Diseases (NIAID) and published in the Journal of Clinical Investigation on February 2, 2021. 

The investigational vaccine, called Ad4-H5-VTN, is a recombinant, replicating adenovirus vaccine designed to spur antibodies to hemagglutinin, a protein found on the surface of influenza viruses that attaches to human cells.

The study authors speculate that replication-competent vector vaccines may have advantages over other types of vaccines because they can express viral proteins at higher levels and for longer durations. 

Additionally, this type of vaccine induces a mucosal immune response critical for limiting the transmission of viruses that infect mucosal tissues.

In this study, the vaccine candidate was administered intranasally (28 study participants), as an oral capsule (10 participants), and via a tonsillar swab (25 participants) to healthy men and non-pregnant women ages 18 to 49 years.

The participants who received the vaccine intranasally or via tonsillar swab showed significantly higher H5-specific neutralizing antibody levels than the group receiving the vaccine capsule orally. 

The participants who received the intranasal vaccine shed viral DNA for two-to-four weeks, but the influenza virus could be cultured for a median of only one day. 

Participants had evidence of H5-specific CD4+ and CD8+ T-cell responses. 

Additionally, volunteers who received the intranasal vaccine had high levels of serum neutralizing antibodies at 26 weeks after vaccination. This level was unchanged at three to five years after a single intranasal dose of the vaccine. 

The duration of viral shedding correlated with a high magnitude of neutralizing antibody response at week 26. The intranasal vaccine also induced a mucosal antibody response in the nose, mouth, and rectum.

According to the study authors, the vaccine platform could be highly adaptable for use against other viruses, including HIV and SARS-CoV-2.

Maryland-based Emergent Biosolutions, Inc developed the investigational vaccine.

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